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猪瘟特异性转移因子的制备及免疫活性鉴定
Preparation and Immune Activity Identification of CSFV-STF
【摘要】 为制备猪瘟特异性转移因子(CSFV-STF)并鉴定其免疫学活性,用猪瘟兔化弱毒和石门系强毒(CSFV-Shimen)配合免疫健康猪,取猪的脾脏,制备猪瘟特异性转移因子,并采用改良白细胞黏附抑制试验检测其免疫活性。结果显示,猪瘟特异性转移因子的LAI%为76.0%,显著高于对照组(P<0.01)。表明猪瘟特异性转移因子具有特异性免疫活性。
【Abstract】 To prepare CSFV-STF and identify its immune activity by centrifugation and ultrafiltration,CSFV-STF was extracted from the porcine spleens,which had been immunized with recombined CSFV-Shimen strain.The immune activity was detected by leukocyte adherence inhibition test.Physicochemical properties and bioactivities of CSFV-STF were accorded with the standard of biological product.The LAI mouse of experimental group was significantly higher than that of control groups(P<0.01).CSFV-STF has a specific immune activity.
【关键词】 猪瘟；
特异性转移因子；
白细胞黏附抑制试验；
【Key words】 Classical swine fever； specific transfer factor； leukocyte adherence inhibition test；
【Key words】 Classical swine fever； specific transfer factor； leukocyte adherence inhibition test；
【基金】 西北农林科技大学博士专项科研基金资助项目
- 【文献出处】 动物医学进展 ,Progress in Veterinary Medicine , 编辑部邮箱 ,2007年02期
- 【分类号】S852.4
- 【被引频次】4
- 【下载频次】221