【摘要】 目的研究大蒜油对胃癌细胞cyclin E表达的抑制作用,探讨大蒜油抗肿瘤作用机制。方法⑴胃腺癌SGC7901细胞在体外常规培养,用免疫荧光染色、流式细胞仪(FCM)检测细胞转化生长因子α(TGFα)及其受体即表皮生长因子受体(EGFR)的表达情况。⑵胃腺癌SGC7901细胞先用无血清培养48h,然后用2.5%低浓度血清培养。在加入TGFα或大蒜油处理,或同时加入TGFα和大蒜油处理后,用免疫荧光染色、FCM检测细胞cyclinE表达的变化。结果⑴胃癌细胞常规培养48h后,TGFα和EGFR表达的阳性率分别为46.80%和57.78%。⑵加入30μg/L TGFα作用5h,cyclin E表达的阳性率增加了7.06%(P<0.001);加入10%大蒜油作用5h,cyclin E表达下降了11.75%(P<0.001);同时加入上述浓度的TGFα和大蒜油作用5h,与单加TGFα处理比较,cyclin E表达下降了17.11%(P<0.01),下降程度大于单加大蒜油引起的下降程度。结论⑴胃腺癌SGC7901细胞表达TGFα和EGFR,具有TGFα自分泌、旁分泌促增殖环路。⑵大蒜油不仅抑制常规培养的胃癌细胞cyclin E表达,而且抑制TGFα刺激的cyclin E表达。提示大蒜油可通过抑制胃癌细胞的TGFα自分泌、旁分泌促增殖环路,发挥抗肿瘤增殖作用。更多还原

【Abstract】 Objective To explore the inhibitory effect of garlic oil on cyclin E expression in gastric adenocarcinoma cells.Methods Human gastric adenocarcinoma SGC7901 cells were cultured routinely and the expressions of transforming growth factor alpha(TGFα)and epidermal growth factor receptor(EGFR)are detected by immunofluorescent staining and flow cytometry.The SGC7901 cells were also cultured with RPMI-1640 without calf serum for 48 h,followed by further culture with RPMI 1640 in the presence of 2.5% calf serum before treatment with TGFα,garlic oil,or their combination,and cyclin E expression of the cells was then detected by immunofluorescent staining and flow cytometry.Results The positivity rates of TGFα and EGFR expressions were 46.80% and 57.78 % respectively in SGC7901 cells cultured routinely for 48 h.The positivity rate of cyclin E expression was increased by 7.06%(P<0.001)in SGC7901 cells treated with 30 μg/L TGFα for 5 h,decreased by 11.75%(P<0.001)following a 5-hour treatment with 10% garlic oil,and decreased further by 17.11%(P<0.001)after treatment with both 30 μg/L TGFα and 10% garlic oil for 5 h.Conclusions The gastric adenocarcinoma SGC7901 cells express TGFα and EGFR and possess TGFα autocrine and paracrine loops to promote cell proliferation.Garlic oil inhibits cyclin E expression in routinely cultured SGC7901 cells and also in TGFα-treated ones,suggesting that garlic oil can inhibit the TGFα autocrine and paracrine loops,which can be one of the pathways of garlic oil to inhibit cancer cell proliferation.更多还原