【摘要】 目的建立整体可视化结肠癌原位动物模型及转移模型,实时观察结肠癌发展、转移的规律。方法将pEGFP-N1表达质粒转染入人结肠癌细胞株SW480中,建立稳定表达绿色荧光蛋白(EGFP)的结肠癌细胞株SW480/EGFP;裸鼠皮下接种SW480/EGFP细胞,利用肿瘤细胞表达EGFP的特点,借助整体荧光成像系统,并利用IPP5.0软件采集、分析结肠癌细胞发出的荧光信号,实时观察结肠癌细胞在裸鼠皮下的生长情况;利用结肠癌外科原位手术移植方法建立结肠癌原位及转移动物模型,应用病理学方法对结肠癌原位及转移动物模型进行评价和鉴定。结果结肠癌细胞SW480/EGFP稳定、高效表达EGFP。皮下接种SW480/EGFP细胞后,可以在动物活体外实时观察肿瘤的皮下生长情况,并进行定量分析;利用外科原位移植的方法建立的结肠癌原位动物模型,成功率达到100%。手术后2周,裸鼠结肠原位成瘤未发现有转移情况,手术后6周,75%裸鼠发生了腹腔转移,50%发生了肝转移。通过病理学方法验证了可视化模型的可靠性。结论整体可视化结肠癌动物原位及转移模型是研究结肠癌发展及转移的可靠有效的观察模型。更多还原

【Abstract】 Objective To establish an animal model visualizing orthotopic growth and metastasis of colorectal cancer.Methods pEGFP-N1 plasmid was transfected into human colorectal carcinoma cell line SW480 so that the resultant SW480/EGFP cells emitted fluorescence that could be detected externally by fluorescence stereo microscope.SW480/EGFP cells were inoculated subcutaneously in nude mice,and the orthotopic tumor growth was evaluated in real time.Whole-body visualization models of orthotopically implanted colorectal carcinoma was established surgically,and the tumor growth and metastasis are evaluated by conventional pathological methods.Results SW480/EGFP cells stably expressed high-levels of enhanced green fluorescent protein.Subcutaneous injection of SW480/EGFP cells resulted in tumor growth in nude mice,and the emitted fluorescence could be quantitatively detected and imaged with fluorescence stereo microscope to visualize real-time tumor growth.Visualization animal model was established successfully with surgical orthotopic implantation(SOI)of the tumor,and all mice survived.After two weeks,all the mice developed colorectal carcinoma without metastasis,but 4 weeks later,75% of the mice developed peritoneal tumor metastasis and 50% had liver metastasis.The whole-body visualization animal model was successfully validated by pathological detection.Conclusion Whole-body visualization model of orthotopic and metastatic tumor growths provides a reliable means for observing the behavior of human colorectal carcinoma and can be helpful to study the growth and metastasis patterns of the cancer.更多还原