【摘要】 目的探讨胃癌组织中Bmi-1基因mRNA表达与胃癌分化、转移及临床预后的关系。方法收集我院2006年5月～2006年11月共42例行胃癌手术切除标本,提取肿瘤及癌旁组织配对标本的总RNA,用RT-PCR方法测定Bmi-1基因表达,并结合临床资料,对Bmi-1基因差异表达与胃癌病人临床表现的相关性进行分析研究。结果42例配对标本分别进行Bmi-1mRNA荧光检测比较,29例标本的肿瘤组织中Bmi-1基因mRNA表达明显高于癌旁正常胃组织。Bmi-1mRNA的表达与胃癌大小、淋巴结转移和浸润深度密切相关(P<0.05),而与患者的性别、年龄、肿瘤分化程度等无关(P>0.05)。并且Bmi-1mRNA阳性表达者生存率明显低于阴性者。结论Bmi-1基因在胃癌组织中表达状态与胃癌的生长和浸润转移关系密切,Bmi-1基因mRNA可望作为胃癌病情发展及指导临床治疗的标记物之一,Bmi-1mRNA的测定有助于判断肿瘤预后。更多还原

【Abstract】 Objective To investigate the association of B cell-specific MLV integration site-1(Bmi-1) mRNA expression level with the differentiation,metastasis and prognosis of gastric carcinoma.Methods Tissue specimens were obtained from 42 patients undergoing surgery for gastric carcinomas.Reverse transcriptional PCR(RT-PCR) was performed for amplification of Bmi-1 mRNA from the 42 tumor tissues and matched adjacent normal tissues,and the differential Bmi-1 mRNA expression was analyzed for its association with the clinical manifestations of the patients.Results Bmi-1 mRNA expression was detected in all the gastric carcinoma tissues and normal tissues adjacent to the carcinoma using fluorescence method,and in 29 cases,Bmi-1 mRNA expression was significantly higher in the tumor tissues than in the adjacent tissues.Expression of Bmi-1 mRNA was highly correlated with tumor size,lymph node metastasis and T classification(P<0.05),but not with the patients’ gender,age,or tumor differentiation(P>0.05).The survival rate was much lower in patients positive for Bmi-1 mRNA expression than in those without Bmi-1 mRNA expression.Conclusions Bmi-1 mRNA expression is correlated to differentiation and metastasis of gastric carcinoma and may facilitate its prognostic evaluation.Bmi-1 may serve as a marker for assessing the progression of gastric carcinoma and provide assistance for clinical therapeutic decisions.更多还原