【摘要】 目的研究慢性粒细胞性白血病(CGL)MICA*008基因与HCMV感染的相关性。方法应用PCR/SSP方法分别检测86例慢性粒细胞性白血病患者和81例无血缘关系的随机健康个体的MICA*008基因,同时用ELISA的方法分别检测随机健康个体和慢性粒细胞性白血病患者接受骨髓移植手术前、后外周血HCMV-IgM抗体。结果①CGL患者组MICA*008基因频率(22.2%)比对照组(34.3%)低(χ~2=4.98,P<0.05);②MICA*008基因阴性个体的HCMV感染率高于阳性个体的HCMV感染率(P<0.05),MICA~*008基因与HCMV感染关系呈中度相关(C=0.5829,0.6142)。结论MICA*008基因阳性个体对HCMV不易感,阴性个体对HCMV易感,即MICA*008基因与HCMV负相关。更多还原

【Abstract】 Objective To investigate the correlation between MHC class I-related chain A (MICA) gene *008 allele and human cytomegalovirus (HCMV) infection. Methods MICA*008 allele was detected in 86 patients with chronic granulocytic leukemia and 81 unrelated normal individuals by way of sequence-specific primers (PCR-SSP). Anti-HCMV IgM was also detected in the sera of these subjects with enzyme linked immunosorbent assay (ELISA). Results MICA*008 allele frequency was lower in patients with chronic granulocytic leukemia than in the control group (22.2% vs 34.3%, χ~2=4.98, P<0.05). The infection rate of HCMV was significantly higher in those individuals with genotype of MICA*008 (-) than in those with MICA*008 (+), and moderate correlation was suggested between MICA*008 and HCMV infection (C=0.5829, 0.6142). Conclusion Individuals with MICA~*008 positivity is not liable to HCMV infection, but those with MICA *008 (-) can be vulnerable to HCMV infection, suggesting an inverse correlation between MICA*008 allele with HCMV.更多还原