【摘要】 目的研究干细胞抗原(Sca-1),CD24和Muc1在静止期乳腺中的表达。方法用Western免疫印迹法检测Sca-1和CD24在6周龄和9周龄大鼠乳腺中的含量变化,制备6周龄、9周龄SD雌性大鼠乳腺标本,切片(4μm),运用免疫组化酶标和免疫组化荧光法对乳腺中Sca-1,CD24,Muc1进行标记。结果Sca-1,CD24在6周龄的大鼠乳腺中的含量略低于9周龄大鼠;Sca-1在乳腺叶间导管、分支导管及腺泡周围表达;CD24在分支导管、乳脂垫及腺泡周围中表达;Muc1在分支导管和叶间导管中表达。结论Sca-1阳性细胞可能代表一类乳腺祖细胞,CD24阳性细胞代表一类乳腺干细胞,而Muc1阳性细胞代表成熟腺上皮细胞。本实验为上述细胞的鉴定提供了一些形态学证据,但需要细胞移植实验进一步实证。更多还原

【Abstract】 Objective To investigate the expressions of the stem cell antigen-1 (Sca-1), Mucin1 (Muc1) and CD24 in quiescent mammary glands of female rats. Methods The expressions of CD24 and Sca-1 were detected in 6-and 9-week-old female rat mammary gland by Western blotting. Sections (4 μm) of 6-and 9-week-old female SD rat mammary gland were prepared to observe the expressions of Sca-1, Muc1 and CD24 by immunohistochemical labeling and immunofluorescence labeling. Results CD24 and Sca-1 in the mammary glands were expressed at lower level in 6-week-old female rats than in 9-week-old female rats. Sca-1 expression was detected in the mammary gland ductus, branching ductus, and areas surrounding the gland alveolus; CD24 was expressed in the mammary gland branching ductus and fat pads, and also the regions surrounding the gland alveolus. Muc1 expression was localized in the mammary gland ductus and branching ductus. Conclusions Sca-1-, CD24-and Muc1-positive cells may represent mammary gland progenitor cells, mammary gland stem cells, and mammary gland mature epithelium cells, respectively. This study provides some morphological evidences for identifiying these cells, but they still need further verifications in cellular transplantation experiments.更多还原