【摘要】 目的:观察自发性高血压大鼠(SHR)下丘脑视上核和室旁核γ-adducin表达的增龄变化,以及高盐摄入的影响.方法:将60只SHR按年龄分幼年组(5wk),成年组(25wk),老年组(49wk),每组20只,测各年龄组SHR尾动脉收缩压;各年龄组随机分为两亚组,分别给予正常盐饮食和高盐饮食喂养3wk;复测血压比较各年龄组正常盐(9g/LNaCl)和高盐(40g/LNaCl)喂养后收缩压的变化;SHR大脑灌注取材,应用免疫组化SABC染色检测SHR下丘脑视上核和室旁核γ-adducin的增龄性表达及高盐喂养的影响.结果:SHR血压随着年龄增加呈递增趋势(P<0.01),高盐喂养能明显升高这种增龄性改变(P<0.01).下丘脑视上核和室旁核中γ-ad-ducin的灰度值均随年龄增长呈递增趋势,即γ-adducin的表达均随年龄增长呈递减趋势.各年龄段内高、正常盐亚组间比较,下丘脑视上核和室旁核中γ-adducin的表达均无统计学差异(P>0.05).结论:SHR血压随着年龄的增长呈逐渐升高趋势,下丘脑视上核和室旁核γ-adducin表达随年龄增长均呈递减趋势,高盐干预对大鼠下丘脑视上核和室旁核γ-addu-cin表达无明显影响.更多还原

【Abstract】 AIM:To examine whether the expression of γ-adducin(one subunit of adducin)alters in the hypothalamic supraoptic and paraventricular nuclei(SON and PVN)of spontaneously hypertensive rats(SHR)with aging,and to evaluate the effect of sodium loading on the expression of γ-adducin in SON and PVN of SHR.METHODS:According to the age of the rats,60 male SHR were divided into 3 groups:infant group(5 wk),adult group(25 wk)and aged group(49 wk)(n=20 in each group).After 1-week normal diet,each group was randomly divided into 2 sub-groups:respectively fed normal-salt(9 g/L NaCl)or high-salt(40 g/L NaCl)rat chow for 3 weeks.We set up a two-phased study:the systolic blood pressure(SBP)of SHR was measured and analyzed by a tail-cuff method before and after sodium loading;the expression of γ-adducin in the SON and PVN of SHR was assessed by immunohistochemical SABC staining.RESULTS:SBP of SHR significantly increased with aging(P<0.01).Compared to SHR with normal-salt diet,SBP of SHR with high-salt diet remarkably increased(P<0.01).The expression of γ-adducin decreased in the SON and PVN of SHR with aging.In the expression of γ-adducin in the SON and PVN of SHR,no significant difference was found between the normal-salt and high-salt sub-groups(P>0.05).CONCLUSION:The blood pressure of SHR increases with aging and high-salt diet.The expression of γ-adducin decreases in the SON and PVN of SHR with aging.There is no significant change in the expression of γ-adducin in the SON and PVN of SHR with short-term sodium loading.更多还原