【摘要】 目的:探讨褪黑素(MT)对大鼠慢性压迫脊髓损伤半胱-天冬氨酸蛋白酶3(Caspase-3)表达和细胞凋亡的影响.方法:将70只大鼠建立慢性压迫损伤动物模型,随机分为MT治疗组(A组)和含50mL/L无水乙醇的生理盐水对照组(B组),于损伤1,4,11,21,35,60和90d取材,采用HE染色观察慢性压迫脊髓损伤脊髓组织病理变化,用免疫组化法染色检测Caspase-3表达阳性细胞,原位末端标记法(TUNEL法)标记凋亡细胞,并用改良的Tarlov评分法和斜板实验观察大鼠脊髓神经功能变化情况.结果:HE染色光镜下观察脊髓组织病理学改变A组明显轻于B组.A,B两组均发现Caspase-3表达及TUNEL标记阳性凋亡细胞,Caspase-3表达和神经细胞凋亡指数均B组>A组(P<0.05),与大鼠脊髓神经功能变化有平行的变化趋势.结论:MT能抑制大鼠慢性压迫脊髓损伤Caspase-3表达和神经细胞凋亡.更多还原

【Abstract】 AIM: To observe the effect of melatonin on expression of caspase-3 and apoptosis in chronic compression spinal cord injury in rats. METHODS: The chronic compression spinal cord injury model was established by compression to the posterior spinal cord in 70 healthy male Wister rats, then the rats were randomly divided into 2 groups: treatment group receiving melatonin (group A) and control group receiving physiological saline containing 50 mL/L ethanol (group B). The rats were killed at day 1, 4, 11, 21, 35, 60, 90 after injury, and then the tissue slices of injured spinal cord were cut for morphological studies by HE staining. The expression of caspase-3 was detected with immunohistochemistry. TUNEL was used to label apoptotic cells. Neurological function changes were assessed with improved Tarlov scores and inclined plane test. RESULTS: The histopathological changes in injured spinal cord tissue observed with HE staining and microscopic examination in group A was distinctly less than those in group B. Expression of caspase-3 and TUNEL-positive cells were both seen in the 2 groups, and the expression of caspase-3 and apoptosis index in group B was higher than those in group A (P<0.05), which was parallel to changing trend of neurological function after chronic compression spinal cord injury. CONCLUSION: Melatonin could suppress caspase-3 expression and apoptosis in chronic compression spinal cord injury in rats.更多还原