【摘要】 目的:研究异丙酚对大鼠局灶性脑缺血再灌注损伤中细胞凋亡的影响.方法:SD大鼠60只,体质量290～310g,随机分为假手术组、异丙酚组和对照组,每组20只.用改良Longa法制成鼠脑缺血/再灌注模型(缺血2h,再灌2h),光镜下观察细胞形态学变化,TUNEL法观察细胞凋亡变化.结果:光镜检查异丙酚组细胞肿胀坏死明显减轻,凋亡细胞及坏死细胞明显减少.再灌2h后对照组凋亡细胞比率、凋亡细胞阳性率、TUNEL阳性率均较假手术组高(P<0.01),异丙酚组凋亡细胞阳性率、TUNEL阳性率较对照组减少(P<0.05).结论:异丙酚能减轻大鼠局灶性脑缺血再灌注损伤,具有抑制神经细胞凋亡坏死的作用.更多还原

【Abstract】 AIM: To study the effects of propofol against cell apoptosis due to ischemia-reperfusion (I/R) injury in rat brain. METHODS: Sixty healthy male SD rats, weighing 290-310 g were selected. Modified Longa model of focal cerebral I/R injury was used (2 h ischemia and 2 h reperfusion).These SD rats were randomly divided into 3 groups: sham operation group, I/R group and propofol pretreatment group. After operation, rats were killed and brains were removed for the detection of apoptotic neurons using TUNEL staining combined with electron microscopic examination. RESULTS: The number of apoptotic neurons of propofol group was significantly less than that of I/R group and sham operation group(P<0.01). The TUNEL positive rate, and apoptosis rate were significantly lower in propofol group than those in I/R group (P<0.05). CONCLUSION: Propofol pretreatment decreases neuronal apoptosis induced by I/R, which relieves the brain injury caused by I/R.更多还原