【摘要】 以二倍体、四倍体和六倍体冰草(P基因组)以及中国春、Fukuho、栽培一粒小麦和硬粒小麦(ABD基因组)等为材料进行RAPD分析,从520个RAPD随机引物中,筛选出2个P基因组特异的RAPD分子标记OPC04和OPP12.利用OPC04和OPP12对小麦族其它基因组植物和8个小麦-冰草二体附加系进行扩增,结果表明OPC04和OPP12在ABD、C、E、AG、I、M、R、S、V、Y等基因组扩增中未出现相应结合位点;而在对8个小麦-冰草二体附加系扩增中均出现此2个特异片段,且扩增稳定、重复性好.进一步表明OPC04和OPP12是冰草P基因组的特异标记,可作为小麦-冰草重组系外源P染色质的鉴定标记之一.更多还原

【Abstract】 A total of 520 decamer oligonucleotides were used to perform random amplified polymorphic DNA(RAPD) analysis on Z804,Z559,Z1750,Chinese Spring,Fukuho,T.monococcum and T.durum.Two P genome specific markers,OPC04 and OPP12 were screened.These two specific primers were then used to amplify on other genome of Triticeae,and 8 wheat-Agropyron cristatum disomic addition lines.The results showed that there is no PCR amplification on ABD,C,E,AG,I,M,R,S,V,Y genome by primer OPC04 and OPP12.However,there are PCR products in all 8 wheat-Agropyron cristatum disomic addition lines.The results further indicated that OPC04 and OPP12 were the specific amplifications for the P genomes.Therefore,OPC04 and OPP12 could be the specific markers on detecting the chromatin of P genome in the wheat-Agropyron progeny.更多还原