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CdCl2对豌豆种子萌发和幼苗生长的影响

Effect of CdCl2 on Seed Germination and Seedling Growth of Pea

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【作者】 杜兰芳; 沈宗根; 王立新; 赵志英; 何天华;

【Author】 DU Lan-fang,SHEN Zong-gen,WANG Li-Xin,ZHAO Zhi-ying,HE Tian-hua (Department of Biology,Changshu College of Physics and Engineering,Changshu,Jiangsu 215500,China)

【机构】 江苏省常熟理工学院生物系; 江苏省常熟理工学院生物系 江苏常熟215500; 江苏常熟215500;

【摘要】 以豌豆为实验材料,采用水培方法研究了Cd2+单盐胁迫对豌豆种子萌发与生长的影响。结果显示:(1)Cd2+质量浓度≤1 mg/L时,促进种子萌发,Cd2+质量浓度达到5 mg/L时抑制种子的萌发。(2)随Cd2+质量浓度的增加Cd2+对幼苗根生长的抑制作用逐渐增强;Cd2+质量浓度≤5 mg/L时,促进茎的生长,≥10 mg/L时,抑制茎的生长;且Cd2+对幼苗根生长的抑制作用大于茎。(3)低浓度Cd2+能促进幼苗叶绿素合成,当Cd2+质量浓度高于1 mg/L时,则对幼苗叶绿素合成有抑制作用,且随Cd2+质量浓度增加叶绿素含量逐渐下降。(4)Cd2+诱发的胚根细胞核、染色体畸变率随着Cd2+质量浓度增加而增大。(5)过氧化物酶(POD)同工酶的活性随着Cd2+质量浓度升高而明显增强,Cd2+质量浓度为1 mg/L时POD活性最强,但当Cd2+质量浓度达10 mg/L时,POD的灰度值明显下降。

【Abstract】 The toxicity damage of CdCl2 on the germination and growth of pea seed were investigated by water culture method.The results showed:(1)at the concentration of the Cd2+ ≤1 mg/L,the seed germination was promoted.While the concentration was increased to 5 mg/L,the seed germination was inhibited.(2)With the concentration of Cd2+ increasing,the inhibition of the growth of seedling was enhanced.At the concentration of the Cd2+ ≤5 mg/L,it promoted the stem growth.But after the concentration of Cd2+ was higher than 10 mg/L,it was obviously to inhibit the stem growth.And this inhibition for seedling root was much stronger than that of stems.(3)At the lower dosage of Cd2+(≤0.5 mg/L),the synthesis of chlorophyll was promoted.With the higher concentration of Cd2+ treatment,it inhibited the synthesis and the content of chlorophyll was decreased with the increasing of the concentration of Cd2+.(4)The nuclear and chromosomal aberration of radicle cell was increased with the increasing of the concentration of Cd2+.(5)There are some significant changes of the POD activation with the increase of concentration of Cd2+.The strongest activation was shown while the Cd2+ was 1 mg/L.However,when the concentration of Cd2+ was arrived 10 mg/L,the grey index of POD was significant decreased.

【关键词】 镉; 豌豆; 种子及幼苗; 萌发; 生长; 过氧化物酶活性;
【Key words】 Cd2+; pea; seed and seedling; germinate; growing; POD activation;
  • 【文献出处】 西北植物学报 ,Acta Botanica Boreali-Occidentalia Sinica , 编辑部邮箱 ,2007年07期
  • 【分类号】X173
  • 【被引频次】38
  • 【下载频次】368
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