【摘要】 以国家二级重点野生保护植物金毛狗为研究对象,采用Knop’s固体与液体培养基培养其成熟孢子,通过显微互动系统详细记录配子体发育的过程,分析其阶段性和多样性,以期为揭示其生殖机理、制定保护对策提供理论依据。结果表明:(1)金毛狗孢子萌发表现为书带蕨型或桫椤型;(2)多数配子体发育无明显的丝状体阶段,而直接进入片状体阶段,这种发育方式在高密度的培养条件下更占优势;(3)金毛狗的配子体发育在2种培养基中表现出较大的差异性和多样性,在液体培养基中,部分丝状体会产生分枝,其中仅有1分枝发育成片状体,但在固体培养基中未观察到此现象;同时,前者的片状体形状表现为圆球形,后者一般表现为短舌状;(4)发育阶段的后期,成熟原叶体都趋向发育成对称的心脏形。更多还原

【Abstract】 The spores of Cibotium barometz,the national second level protection plant,were cultured in Knop’s solution and solid medium respectively.In order to analysis the diversity and the stage and protection,the gametophyte development was observed at various stages under microscope.The results showed:(1) The spore germination belonged to the Vittaria-type or Cyathea-type;(2) Most of the individuals developed into prothallial plate stage directly without the development of filament stage even in crowned cultures;(3) Considerable differences in gametophytic development were observed in two mediums respectively.Some of the filament forked into 2～3 branches and one of them developed into prothallial plate only in Knop’s solution.The prothallial plate is spherical shape in Knop’s solution while it is short tongue-shaped in solid medium;(4) Mature prothalli are found to be symmetrically cordated.更多还原