【摘要】 [目的]从细胞水平研究维生素E琥珀酸酯(VES)对顺铂(CP)引起的前列腺癌(LNCaP)细胞的增殖抑制和凋亡诱导作用,并分析其对前列腺特异性抗原(PSA)表达的影响。[方法]VES、CP及二者联合分别作用LNCaP 24h或48 h,采用MTT法测定其对细胞生长的抑制作用,用荧光染色和透射电镜等形态学方法检测细胞凋亡,并用酶免疫法检测前列腺癌细胞特异性抗原(PSA)的表达情况。[结果]CP、VES和二者合用的IC50分别为1.92 mg.L-1、16.7 mg.L-1和1.02 mg.L-1;CP 4 mg.L-1、VES 25 mg.L-1和二者合用的凋亡率分别为37.2%、57.4%和77.8%;VES可以加强CP抑制LNCaP细胞分泌PSA的作用。[结论]VES可增强CP抗LNCaP细胞的作用。更多还原

【Abstract】 [Objective] To investigate the growth inhibition and apoptosis against cisplatin-induced of vitamin E succinate on human prostate cancer cells and analyze effects of VES on levels of secreted prostate-specific antigen(PSA) in the cells.[Methods] Human prostate cancer cells LNCaP were treated 24 h or 48 h with CP alone,VES alone or a combination of both.The inhibitory effect was measured with MTT method and the cell apoptosis were assessed with the ethidium bromide fluorescence and electron microscope,PSA of LNCaP were examined with ELISA.[Results] IC50 of CP,VES or a combination of both were 1.92,16.7 and 1.02 mg·L-1,respectively,the apoptotic rate was 37.2 %,57.4% and 77.8%,respectively,after treated with CP 4 mg·L-1,VES 25 mg·L-1 and both of them.VES can decrease the PSA level of LNCaP cells and the effect was more obvious after treatment with VES in combination with CP.[Conclusions] VES can enhance the efficacy of anti-LNCaP cells by CP.更多还原