【摘要】 采用热休克法抑制受精卵第一次卵裂诱导虹鳟Oncorhynchus mykiss四倍体,对影响热休克处理效果的3个参数:处理温度(26~31℃)、处理持续时间(3~16 min)、处理起始时刻(3 h 15 min~6 h 50min)进行了研究。结果表明:在授精后3 h 45 min,于31℃下热休克处理5、6、7 min,或30℃下处理7、10 min,或29℃下处理10 min,或28℃下处理16 min,都能成功地诱导出虹鳟四倍体胚胎,四倍体的诱导率为13%~33%,发眼期相对存活率为19.52%~96.54%。更多还原

【Abstract】 Tetraploids in rainbow trout Oncorhynchus mykiss were induced by suppressing the first cleavage by heat shock.The temperature(26-31 ℃),duration(3-16 min)and application time(3 h 15 min-6 h 50 min after egg activation)were investigated during the heat shock.The shock medium intensity including 31 ℃ for 5,6,and 7 min,30 ℃ for 7,and 10 min,29 ℃ for 10 min,and 28 ℃ for 16 min,and 3 h 45 min after fertilization were found to be the suitable treatment for induction of the tetraploidy rainbow trout with the tetraploid rate of 13%-33%,and the survival of 19.52% to 96.54%(relative to that of the diploid control)at the eyed stage.更多还原