【摘要】 从乐清湾红树林中分离获得2株产淀粉酶的真菌,经初步鉴定为曲霉属菌株,暂命名为曲霉5#和曲霉34#。对它们的生长温度、固体发酵时的产酶条件以及淀粉酶特性进行了初步研究。该研究结果表明,曲霉5#和曲霉34#的最适生长温度分别为30~35℃和25~30℃,最高生长温度分别为40℃和35℃,在10℃下均能生长。对两株菌进行固体发酵时其最佳培养时间为96 h。两株菌所产淀粉酶的最适pH值为7.0,最适反应温度为40℃;淀粉酶在50℃下仍能保持活力,60℃下酶活力开始下降,80℃时酶几乎失活;离子对淀粉酶的激活或抑制作用也相似。更多还原

【Abstract】 Two strains of amylase-excreting fungi,temporarily assigned to Aspergillus sp.5# and Aspergillus sp.34#,were screened from the mangrove in the Yueqing Bay,and the conditions of their growth and amylase excretion as well as the characteristics of the excreted amylases were studied.The optimum growth temperature is 30～35℃ and 25～30℃ and the highest growth temperatuer is 40℃ and 35℃ respectively.They can grow in the temperature below 10℃.The optimum conditions of the amylase excretion is as following: 96 h,pH 7.0 and 40℃.The activities of the two amylases are still high at 50℃,decrease at 60℃ and deactivaed at 80℃.The activation and inhibition effects of ions on the two amylases are similar.更多还原