【摘要】 目的探讨Kiss-1在雌性性早熟大鼠不同时期下丘脑中mRNA的表达。方法正常5日龄清洁级Sprague-Dawley雌性大鼠40只,随机分为4组,对照1组(正常青春前期),对照2组(正常青春期早期),模型1组(青春期早期),模型2组(青春期中期),每组10只,达那唑300μg皮下注射以诱导真性性早熟。半定量RT-PCR法检测对照1组,对照2组,模型1组,模型2组的Kiss-1 mRNA的表达。结果与对照1组相比,模型1组和模型2组下丘脑Kiss-1 mRNA水平分别增加了1.4和2.8倍,差异具有显著性(P<0.05)。模型2组大鼠下丘脑Kiss-1 mRNA表达水平高于模型1组,差异有显著性(P<0.05)。对照2组与模型1组的Kiss-1 mRNA的表达差异无显著性(P>0.05)。结论Kiss-1 mRNA的表达与生长发育的时期密切相关,提示Kiss-1可能与真性性早熟的发生有关。更多还原

【Abstract】 Objective To investigate the gene expression of Kiss-1 in the hypothalamus of true precocious female rats at various stages of development. MethodsForty 5-day-old normal female Sprague-Dawley rats were randomly assigned into four groups of 10 rats: Control group 1, Control group 2, Model group 1 and Model group 2. The rats from the two model groups were injected with 300 μg of danazol at 5 days of age to induce true precocious puberty. The two control groups were injected with normal saline instead. For the determination of Kiss-1 mRNA expression in the hypothalamus, the rats of the Model group 1 were sacrificed during the first diestrus (early puberty) and meanwhile the rats of the Control group 1 were sacrificed when they were at prepuberty; the Control group 2 rats were sacrificed at the first diestrus (early puberty); the rats of the Model group 2 were sacrificed during the second diestrus (middle puberty). The expression of Kiss-1 mRNA in the hypothalamus in the four groups was detected using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). ResultsKiss-1 mRNA expression in the hypothalamus in Model group 1 and Model group 2 increased by 1.4-fold and 2.8-fold, respectively, compared with that of Control group 1 (P<0.05). Model group 2 showed significantly higher Kiss-1 mRNA levels than Model group 1 (P<0.05). There were no statistical differences in the Kiss-1 mRNA expression between Control group 2 and Model group 1. ConclusionsGene expression of Kiss-1 is associated with the developmental period of true precicous puberty, suggesting that Kiss-1 might play a role in the pathogenesis of this disorder.更多还原