【摘要】 优化制备维生素C脂质体的配方,采用薄膜分散—超声乳化法制备。以包封率为考察指标,经正交优化选择,确定脂质体的最佳配方:卵磷脂/胆固醇/维生素E的摩尔比为4∶2∶0.34;维生素C的浓度为3.3 mg/mL;PBS缓冲液的pH值为7.93;成膜时所用氯仿的量为30 mL,此时包封率可达58.32%。用扫描电子显微镜观察脂质体的结构,平均粒径为310 nm。更多还原

【Abstract】 To optimize the preparation formulation of vitamin C liposomes,the liposomes were prepared by film dispersion-ultrasonic emulsify method,taking entrapment efficiency as index,and designing an orthogonal experiment.The optimal formulation were: phosphatidylcholine / cholesterin / vitamin E(molar ratio)4∶2∶0.34;vitamin C concentration:3.3 mg/mL;PBS buffer pH value:7.93;chloroform for film forming :30 mL.Under the formulation the entrapment efficiency was 58.32%.The structure of liposome was observed under the scan electronic microscope.The main diameter of the liposome particles was 310 nm.更多还原