【摘要】 为了研究温度、铜离子对鲤鱼血清热应激蛋白(HSP70)合成的影响,探讨HSP70的表达机制,以健康1龄鲤鱼(Cyprinus carpio)为受试生物,10℃下驯养,经过24 h不同温度(10℃,20℃,24℃,28℃)和系列铜离子浓度(0,10,50,100,300,500μg/L)暴露,采用双抗体夹心酶联免疫吸附剂检测法(enzyme-linked immunosorbnent assay,ELISA)检测鲤鱼血清HSP70合成水平,原子吸收光谱法(atomic absorption spectroscopy,AAS)检测血清铜离子含量.结果表明:20℃,24℃热冲击组鲤鱼血清HSP70合成水平高于10℃组,与10℃组相比,差异极其显著(P<0.01);铜离子能够诱导鲤鱼血清HSP70表达,呈现明显的剂量效应关系,热冲击与铜离子具有联合效应.更多还原

【Abstract】 To determine the effects of temperature and copper on carp(Cyprinus carpio) serum HSP70,after acclimation at 10℃,healthy carps were exposed to combinations of three temperature levels(10℃,20℃,24℃) and six copper concentrations(0,10,50,100,300,500 μg/L) for 24 h.HSP70 expression relative to the controls was analyzed by sandwish ELISA(enzyme-linked immunosorbnent assay,ELISA);Copper concentrations in fish serum were measured by atomic absorption spectroscopy(AAS).The results showed that the HSP70 expression in carp serum under 20℃,24℃ are both much higher than that under 10℃(P<0.01).Observed the copper could induce HSP70 expression in carp serum with significant logarithmic dose-effect relationship.There was strong combined effect of temperature and copper on HSP70 expression in this study.更多还原