【摘要】 采用共混法制备丝素-壳聚糖共混膜,并测定其力学性能,结果表明各种配比的丝素-壳聚糖共混膜均具有良好的机械性能,能够克服纯丝素膜刚而脆的弱点。扫描电镜显示纯丝素膜SF、共混膜SCP1表面致密光滑,SCG2、SCP2两种共混膜都有明显突起,而共混膜SCG1的表面凹凸不平。细胞培养结果显示,SCG1、SCG2、SCP1和SCP2等4种共混膜的细胞毒性都在0、1级,细胞增殖率在85%~105%之间,细胞附着率在24 h的达到90%以上,说明各种配比的丝素-壳聚糖共混膜都具有良好的细胞相容性,基本符合生物材料的应用要求。更多还原

【Abstract】 Silk fibroin-chitosan blend membranes were prepared by mixing method and the mechanics of blend membranes was measured.The result shows that the blend membranes have good mechanical properties and can conquer the weakness of silk membranes,stiffness and brickleness.The observation of SEM shows that the surfaces of SF and SCP1 are close and smooth,while those of SCG2 and SCP2 have obvious protuberances.The surface of SCG1 is disorder and has deep protuberance and hollow.The tests of cell culture show that cell cytotoxicity of SCG1,SCG2,SCP1 and SCP2 are in 0 or 1 grade,cell relative proliferation rates of SCG1,SCG2,SCP1 and SCP2 are between 85% and 105% and the attachment ratios of SCG1,SCG2,SCP1 and SCP2 are more than 90%.It shows that silk fibroin-chitosan blend membranes of all combinations have good cell compatibility and meet the requirements for applied biomaterial.更多还原