【摘要】 目的:探讨用1,25二羟基维生素D3[1,25(OH)2VitD3]预干预对大鼠辅助性T细胞(Th1)免疫应答中脾CD4+CD25+调节性T细胞及其特异性转录因子Foxp3基因表达的影响。方法:采用近交系大鼠,随机分为对照组、脂多糖(LPS)组和VitD3组。分别给予对照组和LPS组赋形剂,VitD3组用1,25(OH)2VitD3(0.25μg/只.d-1),连续灌胃14 d。第15天LPS组和VitD3组腹腔注射致死剂量LPS(10 mg/kg)。6 h后处死,用流式细胞仪检测脾CD4+CD25+调节性T细胞比例,用Realtime RT-PCR检测脾中Foxp3 mRNA的表达。结果:1,25(OH)2VitD3明显上调大鼠Th1免疫应答中脾CD4+CD25+调节性T细胞的比例及其特异性转录因子Foxp3 mRNA的表达。结论:1,25(OH)2VitD3预干预对大鼠Th1免疫应答中脾CD4+CD25+调节性T细胞及其特异性转录因子Foxp3的基因表达有显著影响。1,25(OH)2VitD3有可能通过促进CD4+CD25+调节性T细胞的发育,发挥其免疫调节作用。更多还原

【Abstract】 Objective: To investigate the effect of 1,25(OH)2 VitD3 on CD4+,CD25+ regulatory T cells and its specific transcription factor Foxp3 in rat spleen in Th1 immune reactioin. Methods: Lewis rats were randomly divided into 3 groups.VitD3 group was fed with 1,25(OH)2 VitD3(0.25 μg per rat a day) for 14 days.The control group and LPS group administered the isotonic NaCl in the same volume for the same long time.LPS(10 mg/kg) was administered intraperitoneally in LPS group and VitD3 group on the 15th day,and then the animals were sacrificed 6 hours later.The percentage of CD4+CD25+ regulatory T cells in lymphomonocytes in rat spleen was analyzed by Flow cytometry.The expression of Foxp3 gene in rat spleen was detected by Realtime RT-PCR.Results: 1,25(OH)2 VitD3 increased the percentage of CD4+CD25+ regulatory T cells in lymphomonocytes in rat spleen significantly.1,25(OH)2 VitD3 also up regulated the expression of Foxp3 gene markedly.Conclusion: 1,25(OH)2 VitD3 has a great influence on CD4+CD25+ regulatory T cells and its specific transcription factor Foxp3.1,25(OH)2 VitD3 may enhance the development and function of CD4+CD25+ regulatory T cells either directly or indirectly.更多还原