【摘要】 为了解决抑肽酶制备过程中的高污染、高成本、吸附剂使用条件苛刻等问题,本试验采用化学改性与修饰壳聚糖为载体,共价法偶联牛胰蛋白酶,制成抑肽酶亲和吸附剂,将其直接亲和层析牛肺提取液,分离纯化高比活抑肽酶。抑肽酶抑制比活单位(BAEE/mg)为3 936.46,酶活性回收率70.81%,最后的纯化倍数达到114.37倍。更多还原

【Abstract】 The trypsin was covalently linked with the chemical modified chitosan and used to purify aprotinin from the crude extract of cattle lungs by affinity chromatography in order to shun environmental pollution, harsh usable condition and reduce the cost of production.It was shown that the specific activity of immobilized trypsin on chitosan was 3 936.4 6BAEE/mg,and the activity recovery of trypsin was 70.81%.The activity rate of aprotinin is 114.37 times higher than that of the tissue extrac.更多还原