【摘要】 目的通过观察血管活性肠肽(vasoactive intestinal peptide,VIP)促进人脐静脉血管内皮细胞(human umbilical veinvessel endothelial cells,HUVECs)的增殖以及刺激VEGF的合成情况,探讨VIP在创伤组织修复过程中的生物学效应。方法体外培养HUVECs细胞系,分为有血清培养组和无血清培养组,这两组分别加入VIP和PBS进行干预,应用MTT法测定HU-VECs的增殖情况,以酶联免疫吸附法(ELISA)检测血管内皮细胞生长因子(VEGF165)在HUVECs的合成。结果VIP能促进血管内皮细胞的增殖,且两组都有VEGF生成,但VIP组VEGF的量显著高于PBS组。结论VIP能促进HUVECs的增殖以及增强血管内皮细胞内VEGF的合成和分泌,在创伤组织修复过程中可能发挥促新生血管形成的作用。更多还原

【Abstract】 Objective To investigate the biological effect of VIP in the process when the wound tissue was healing,by observing the proliferation of HUVECs activated by VIP and the synthesis of vascular endothelial growth factor in HUVECs which had been cultured in vitro.Methods HUVECs had been cultured in vitro and classified two group which were serum-supplemented medium group and serum-free medium group,then both groups were added VIP or PBS respectively.The proliferation of HUVECs were detected with MTT and the synthesis of vascular endothelial growth factor in human umbilical vein endothelial cells were quantificated with enzyme-linked immunosorbent assay(ELISA).Result The synthesis of vascular endothelial growth factor in both group were observed,but the quantity of vascular endothelial growth factor in the VIP-group were higher than in the PBS-group,and VIP could promote the proliferation of human vein vessel endothelial cells in the two groups.Conclusion Vasoactive intestinal peptide could promote the proliferation of HUVECs and increase the synthesis of the vascular endothelial growth factor in HUVECs,and could have the effect of increase angiogenesis in the process of the wound tissue.更多还原