【摘要】 利用RAPD(随机扩增多态)标记分析湖内鲤、鲫群体遗传多样性,从40个随机引物中各筛选出8个引物适合鲤、鲫群体RAPD扩增。在鲤群体中,共检测出60条带,其中多态性带42条,多态位点比率为70.00%;而在鲫群体中,共检测出61条带,其中多态性带40条,多态位点比率为65.57%。用POPGENE软件分析实验数据,结果显示:湖内鲤群体的遗传多样性水平(He=0.230 1,H0=0.391 0)和鲫群体的遗传多样性水平(He=0.218 6,H0=0.375 8)都较高,都有较大的遗传变异。而在鲤、鲫群体内,雌性群体与雄性群体相比较,其各自的遗传多样性很接近。更多还原

【Abstract】 The colonial genetic variation in Cyprinus carpio and Carassius auratus was analyzed with RAPD technique.Eight primers were screened out from 40 random primers on the genomic DNA in Cyprinus carpio.A total of 60 bands were generated,of which polymorphic bands were 42,with the percentage of polymorphism being 70.00%.Using the 8 primers for genomic DNA of Carassius auratus,61 bands were generated,of which 40 were polymorphic,with the percentage of polymorphism being 65.57%.As analyzed by software POPGENE,the colonial genetic diversity was both high in Cyprinus carpio(He=0.230 1,H0=0.391 0) and Carassius auratus(He=0.218 6,H0=0.375 8).However,in the population of Cyprinus carpio and Carassius auratus,the colonial genetic diversity was very similar in females and males.更多还原