【摘要】 以酶抑制动力学为基础,推导了竞争性、非竞争性、混合型3种抑制类型的抑制率达到50%时的抑制剂浓度(IC50)的计算方法,计算得到了肉桂酸、肉桂醛和苯甲酸等多种酪氨酸酶抑制剂的IC50值。比较竞争性、非竞争性、混合型3种抑制类型IC50计算值和实验值,数据非常相近,平均误差分别为3.7%、2.4%、4.6%,结果表明IC50值的计算方法是可靠的。更多还原

【Abstract】 Calculating methods for inhibitor concentration leading to achieve 50% inhibition(IC50)of competitive inhibitor,noncompetitive inhibitor and mixed inhibitor were derived on the basis of kinetics of enzyme inhibition.These methods were applied to calculate IC50 values of some tyrosinase inhibiting compounds,such as cinnamic acid,cinnamaldehyde and benzoic acid et al.The calculation results of competitive inhibitor,noncompetitive inhibitor and mixed inhibitor are very close to experimental data,and the average errors are 3.7%,2.4%,4.6% respectively.Such results indicates that the calculation methods are reliable.更多还原