【摘要】 利用胃蛋白酶从草鱼鱼鳞中提取酶溶性胶原蛋白(PSC)。并用氨基酸自动分析仪、SDS-PAGE电泳、DSC、FT-IR和圆二色谱对草鱼鱼鳞胶原蛋白的氨基酸组成、相对分子量、变性温度和结构进行了研究。试验结果表明:所提草鱼鱼鳞蛋白为典型的I型胶原蛋白;且至少含有2条α链,α1和α2链;DSC分析表明其变性温度为37.1℃。更多还原

【Abstract】 Pepsin-soluble collagen(PSC) was extracted from scales of grass carp(Ctenopharyndogon idellus) by pepsin.The basic properties of the extracted collagen were characterized through SDS-PAGE,FT-IR,amino acid analyzer,CD and DSC.The results indicated that the extracted collagen comprised at least two different α chains,α1 and α2,and was classified as type Ⅰ collagen;Denaturation temperature(Td) of the lyophilized PSC was 37.1 ℃ by DSC.更多还原