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羊草与灰色赖草杂种F₁再生体系的建立

Study of Plant Regeneration in the Hybrid F₁ Between Leymus chinensis and Leymus cinereus

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【Author】 HOU Jian-hua1,ZHANG Ying1,YUN Jin-feng2*,ZHANG Zhi3,GAO Zhi-jun2 (1.College of Agronomy,Inner Mongolia Agriculture University,Hohhot,Inner Mongolia Autonomous Region 010018,China;2.College of Ecology and Environment,Inner Mongolia Agriculture University,Hohhot,Inner Mongolia Autonomous Region 010018,China;3.Inner Mongolia Yili Industrial Group Co.Ltd.Hohhot,Inner Mongolia Autonomous Region 010080,China)

【机构】内蒙古农业大学农学院；内蒙古农业大学生态环境学院；内蒙古伊利实业集团股份有限公司；内蒙古农业大学农学院呼和浩特010018；呼和浩特010018；呼和浩特010019；呼和浩特010080；

【摘要】对羊草(Leymus chinensis)与灰色赖草(Leymus cinereus)杂种F1幼穗进行离体培养,建立了杂种F1植株再生体系。结果表明:不含任何激素的MS培养基是最佳的分化培养基,分化成苗率为66%;2,4-D对愈伤组织的诱导及其质地的改造有重要作用;3.0 mg/L 2,4-D+MS培养基愈伤组织诱导率最高,为68%;2.0 mg/L 2、4-D+MS诱导的愈伤组织胚性最强;诱导率与分化率没有直线对应关系;再生体系的建立为杂种F1育性恢复的研究奠定了基础。更多还原

【Abstract】 The study was undertaken to induce F1 young spikes between L.chinensis and L.cinereus to develop a system of plant regeneration.Results indicate that hormone-free MS medium was the best in the differentiation at a rate of 66%,while 2、4-D was important for callus induction and quality improvement.The highest rate of callus induction was 68% in the medium of 3.0 mg/L 2、4-D+MS.Embryogenic calluses were obtained in the medium of 2.0 mg/L 2、4-D+MS.There was no lineal correlation between callus induction and plant regeneration frequency.The development of the plant regeneration would lay a foundation for the fertility restoration in F1.更多还原