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温州水牛线粒体DNA D-loop遗传多态性分析
Analysis of Genetic Diversity on mtDNA D—loop region in Wenzhou Buffalo
【摘要】 以30头温州水牛为研究对象,对每个个体的线粒体DNA(mtDNA)D—loop区915 bp全序列进行了分析研究,结果共检测到14种单倍型,50个核苷酸多态位点,其中出现核苷酸转换48个,颠换2个。温州水牛mtDNA D—loop区核苷酸多样度(π值)为0.0184,单倍型多样度(H)为0.8575,表明温州水牛线粒体DNA遗传多样性丰富。NJ聚类分析表明,温州水牛有2个母系起源。
【Abstract】 The complete mitochondrial DNA D—loop sequences,915 bp in length,in 30 Wenzhou buf- falo samples were analyzed.The results revealed 14 mitochondrial haplotypes and 50 polymorphic sites with 48 transitions and 2 transversions.The nucleotide diversity (πvalue) and haplotype diversity (H val- ue) estimated from mtDNA D—loop region were 0.0184 and 0.8575,respectively,indicating rich mito- chondrial genetic diversity in Wenzhou breed.The NJ clustering analysis indicated that there were two ma- ternal origins in Wenzhou breed.
【Key words】 Wenzhou buffalo; MtDNA D—loop; Genetic diversity; Haplotype; Origin;
- 【文献出处】 中国牛业科学 ,China Cattle Science , 编辑部邮箱 ,2007年01期
- 【分类号】S823
- 【被引频次】10
- 【下载频次】208