【摘要】 目的:探讨兔动脉粥样硬化模型中一氧化氮(NO)和血浆纤溶酶原激活物抑制物-1(PAI-1)的表达及阿托伐他汀的干预作用。方法:24只雄性日本大耳白兔随机分为高脂饮食组(A组)、高脂饮食+阿托伐他汀组(B组)和正常饮食组(C组),每组8只。各组分别在喂养16周末处死,ELISA法检测NO和PAI-1。全自动生化分析仪检测血清脂蛋白的表达水平。结果:NO的表达水平B组显著高于A组和C组(P<0.01),A组与C组比较差异无显著性(P>0.05)。PAI-1的表达水平B组低于A组和C组(P<0.01),A组与C组比较差异无显著性(P>0.05)。A、B、C三组总胆固醇(TC)水平分别为(23.51±10.58)、(14.27±3.51)和(1.36±0.33)mmol·L-1,三组间两两比较差异有显著性(P<0.05)。A、B、C三组低密度脂蛋白(LDL)水平分别为(21.39±10.00)、(14.23±4.01)和(0.72±0.35)mmol·L-1,三组间两两比较差异有显著性(P<0.05)。甘油三酯(TG)水平三组间比较差异无显著性(P>0.05)。结论:高脂饮食对兔动脉粥样硬化模型中NO和PAI-1表达水平无明显影响,阿托伐他汀干预使NO表达水平升高,使PAI-1表达水平降低,有利于抑制动脉粥样硬化的进程。更多还原

【Abstract】 Objective To investigate the expressions of nitric oxide (NO) and plasminogen activator inhibitor type-1(PAI-1)in rabbit models of atherosclerosis and the interference effect of atorvastatin. Methods Twenty four male Japanese rabbits were divided into 3 groups (8 in each group): high-fat diet group(group A),high-fat plus atorvastatin group (group B) and normal diet group(group C).The expressions of NO and PAI-1 were detected with ELISA assay.Plasma lipid was detected with automatic analysis divice. Results The espression of NO in group B was significantly higher than those in groups A and group C (P<0.01); there was no significant difference between groups A and C (P>0.05).The expression of PAI-1 in group B was significantly lower than those in groups A and C,there was no significant difference between groups A and group C (P>0.05).The levels of serum TC in groups A,B,and C were (23.51±10.58),(14.27±3.51)and(1.36±0.33)mmol·L-1, respectively; the levels of serum LDL in groups A,B,and C were (21.39±10.00),(14.23±4.01)and(0.72±0.35)mmol·L-1;there were significant differences between three groups (P<0.05),while TG had no significant difference between three groups (P>0.05).Conclusion High-fat diet has no significant effect on the expressions of NO and PAI-1 in atherosclerosis rabbits .Atorvastatin can increase the expression of NO and decrease the expression of PAI-1,and can inhibit the progression of atherosclerosis.更多还原