【摘要】 目的:观察甘氨酸(Gly)对梗阻性黄疸大鼠肠道屏障的影响。方法:选择成年雌性Wistar大鼠60只,随机均分为3组:假手术组(A)、胆总管结扎组(B)和胆总管结扎+甘氨酸组(C)。手术结扎胆总管复制梗阻性黄疸模型。甘氨酸用药方法为术前5 d及术后自由饮用含5%甘氨酸的水溶液,术后第21天处死大鼠,检测血清胆红素水平和肠道匀浆组织NO含量,并观察肠道形态学变化和黏膜厚度、绒毛高度。结果:与假手术组(A)比较,胆总管结扎组(B)血清胆红素水平、肠组织中NO含量均明显增高(P<0.01),肠绒毛高度、黏膜厚度均明显降低(P<0.01),肠道组织结构损害较重。胆总管结扎+甘氨酸组(C)肠组织中NO水平较胆总管结扎组(B)明显降低(P<0.01),肠道组织结构损害较轻。虽然胆总管结扎+甘氨酸组(C)胆红素水平较假手术组(A)增高(P<0.01),但是肠组织中NO水平、肠绒毛高度、黏膜厚度均无显著变化(P>0.05)。结论:梗阻性黄疸时,NO水平增高,肠道屏障功能受到严重破坏,甘氨酸能降低内毒素的生物学效应,减少NO的产生,有助于梗阻性黄疸时的肠道屏障功能保护。更多还原

【Abstract】 Objective To explore the effect of glycine on the intestinal barrier of rats with obstructive jaundice.Methods The male Wistar rats weighing 220—260g were randomly assigned to three groups(n=20): sham operation group(A),common bile ligation gruop(B),common bile duct ligation plus glycine group(C).Except sham operation group(A),rat models with obstructive jaundice in the other groups were set up by double ligation of the common bile duct.The rats in group C was fed with 5% glycine solution for 5 d.From the second day after the operation,the rats in group C were fed according to the preoperative standard.On the 21th day after operation,the rats were killed and serum bilirubin(BIL) and the amount of nitric oxide(NO) in intestinal tissue were determined,the morphological changes of the intestine were observed,the villus height and mucosal thickness were measured.Results Compared with group A,the serum BIL and the amount of NO in intestinal tissue were increased and the villus height and mucosal thickness were reduced in group B(P<0.01).As compared with group B,the amount of NO were reduced in group C(P<0.01),the architecture distortion in the intestinal tissue was alleviated,an increase was noted in the villus height and mucosal thickness(P<0.01).In group C,the BIL increased(P<0.01),however there were no significant differences of the amount of NO,villus height,mucosal thickness(P>0.05) compared with group A.Conclusion Glycine can decrease the level of NO in the intestinal tissue and improve the damaged intestinal barrier obviously.更多还原