【摘要】 目的:探讨姜黄素(curcumin)对人前列腺癌PC-3M细胞生长抑制及凋亡调控的作用。方法:按姜黄素浓度分为10、20、30和40μmol.L-14个处理组和对照组(未加姜黄素),处理PC-3M细胞不同时间后,倒置显微镜观察细胞形态;采用MTT法检测细胞的增殖抑制情况;荧光染色法检测细胞凋亡;流式细胞仪(FCM)进行细胞周期时相分析;免疫组化SP法检测细胞内caspase-3蛋白的表达水平。结果:与对照组比较,姜黄素处理组可使细胞明显变圆、体积缩小、脱壁细胞增多;MTT法检测显示,姜黄素处理组随着浓度的增加和作用时间延长,对PC-3M细胞的增殖抑制作用增强,并呈时间、剂量依赖性(P<0.01);荧光显微镜下部分细胞发生凋亡形态学改变,对照组和4个姜黄素不同浓度处理组凋亡率分别为(9.83±1.53)%、(19.50±1.00)%、(24.83±2.52)%、(30.17±2.08)%和(38.50±2.65)%;流式细胞术显示,停滞在S、G2/M期细胞增加,而G0/G1期细胞减少;免疫组化结果显示,随着姜黄素浓度增加,caspase-3蛋白表达增强,呈剂量依赖性(P<0.01)。结论:姜黄素对人前列腺癌PC-3M细胞的生长具有明显抑制作用。上调caspase-3蛋白的表达,诱导细胞凋亡可能是其作用机制之一。更多还原

【Abstract】 Objective To investigate the regulatory effect of curcumin on proliferation inhibition and apoptosis in human prostate carcinoma PC-3M cells.Methods The PC-3M cells were divided into 4 treatment groups(10,20,30 and 40 μmol·L-1 curcumin) and control group.The morphological alterations of PC-3M cells after treated for different time were observed by using inverse microscopy,the inhibition of cell proliferation was detected by MTT assay,the cell cycle phase was analyzed by flow cytometry,the apoptosis was inspected by acridine orange-ethidium bromide fluorescent staining method,the protein level of caspase-3 in carcinoma cells was observed by SP immunohistochemistry.Results After being treated with curcumin,PC-3M cells grew round and small obviously and were against the wall under the inverse microscope.with the increasing of the concentration of cucurmin and the elongation of the treated time,the PC-3M cell growth was inhibited,the inhibition of proliferation of PC-3M cells increased in a time-and dose-dependent manner(P<0.01);partial cells presented the morphological changes of apoptosis under the fluorescent microscope,the apoptotic rates of the control group and 4 treatment groups were(9.83±1.53)%,(19.50±1.00)%,(24.83±2.52)%,(30.17±2.08)% and(38.50±2.65)%,respectively;the cell number in S and G2/M phase were increased,the cell number in G0/G1 phase was decreased;with the increasing of concentration of curcumin,the caspase-3 expression was increased in a dose-dependent manner(P<0.01).Conclusion Curcumin could significantly inhibit the growth of PC-3M cells,the induction of apoptosis through up-regulating caspase-3 is probably one of its molecular mechanisms.更多还原