【摘要】 通过表面接枝改性法对上转换发光材料Na[Y0.57Yb0.39Er0.04]F4进行表面修饰羧基的研究,并对影响因素如反应时间、缓冲溶液以及修饰剂的加入量进行了系统的实验分析,解决了上转换发光材料作为荧光标记物使用时无法直接共价固定生物活性分子的问题.实验结果表明,当反应时间为4h,缓冲溶液为pH=9.0,0.50mol·L-1的PB缓冲溶液,缓冲溶液的使用量为50mL,修饰剂的加入量为氨基化材料质量的12倍时,上转换发光材料表面成功修饰羧基的量达到最大.更多还原

【Abstract】 The surface of an up-conversion luminescence material was modified with carboxyl group in the way of grafting modification. The influencing factors including reaction time, buffer solution and finishing agent’s mass were investigated by experiment. This modification method has resolved the problem that when an up-conversion luminescence material is used as fluorescence labeling, it cannot covalently fix biological activity molecules directly. The experimental results show that when the reaction time is 4 h, the PB buffer solution is pH=9.0 and 0.50 mol·L-1, the volume of adding the buffer solution is 50 mL, and the mass ratio of finishing agent to original materials is 12, the surface of an up-conversion luminescence material is mostly modified with activity carboxyl group.更多还原