节点文献
葡萄糖氧化酶在管状空心SiO2载体上的固定化
Hollow silica nanotubes for immobilization of glucose oxidase enzyme
【摘要】 以针状CaCO3为无机模板,制作成新型固定化材料管状空心SiO2载体,用来固定葡萄糖氧化酶(GOD)。研究了pH值,温度和载体与GOD的配比等对固定化酶活的影响。所得的最佳工艺条件为:最佳载体和游离酶配比为0.6 g/mL;固定化酶的最适pH值为5.2;最佳反应温度为32℃。和游离酶相比无论操作性还是稳定性都有提高。
【Abstract】 Porous hollow silica nanotubes(PHSNTs) have been synthesized via a sol-gel route using nano-sized needle-like CaCO3 as the inorganic template and employed as a support for immobilization of Glucose Oxidase enzyme(GOD) biocatalyst.The effect of varying factors such as phosphate buffer pH,immobilization temperature and ratio of carrier to free GOD(g/mL) on the activity of immobilized GOD are discussed.The optimum conditions were found to be as follows: ratio of carrier to free GOD(g/mL) 0.6 g/mL,phosphate buffer pH 5.2,immobilization temperature 28 ℃.The results show that under optimized conditions the efficacy and stability of GOD are improved relative to the free enzyme.
【Key words】 enzyme immobilization； glucose oxidase enzyme(GOD)； hollow silica nanotubes； needle-like CaCO3；
- 【文献出处】 北京化工大学学报(自然科学版) ,Journal of Beijing University of Chemical Technology(Natural Science Edition) , 编辑部邮箱 ,2007年04期
- 【分类号】Q814.2
- 【被引频次】7
- 【下载频次】258