【摘要】 以满天星试管苗叶片为材料,进行满天星基因组DNA提取及RAPD扩增条件优化。结果表明,采用CTAB法提取的DNA质量较高,适宜于RAPD分析;RAPD扩增最佳反应体系为25μL:14.17μLddH2O,150μmol/LdNTP,1.5μmol/LMgCL2,2.5μLBuff-er,0.3μmol/L引物,10ngDNA模板,1UTaqDNA聚合酶。扩增反应程序为:94℃预变性5min,94℃变性1min,36℃退火1min,72℃延伸1.5min;45个循环;最后72℃延伸7min。更多还原

【Abstract】 Used fresh leaves of Gypsophila elegans L. as matenals.The method of genomie DNA extraction and the optimization of RAPD analytic conditions were studied in Gypsophila elegans L.The result showed that the high-quality genomie DNA was obtained by the CTAB method.The optimal PCR system for RAPD analysis was as follows:14.17μL dd H2O,150μmol/L dNTP,1.5μmol/L Mg2+,2.5μLBuffer,0.3μmol/L random primer,10ng template,1U Taq polymerase in 25μL reaction system.The program of amplifying reaction was as follows: After pre-denaturing at 94 ℃for 5 min,under the condition of denaturing at 94℃ for 1 min ,annealing at 36℃ for 1 min,extension at 72℃ for 1.5 min, amplifying for 45cycles,and extension at 72℃ for 7 min at last 。更多还原