【摘要】 利用H5亚型禽流感病毒血凝素抗原快速检测试剂“H5-HA(Ag)Dot-ELISA(H5-Dot)”对来自陆禽、水禽的484份气管拭子、泄殖腔拭子和粪便拭子标本进行检测,结果:①不同采集方式的H5N1阳性标本的检出率高低有别,气管拭子的检出率最高,泄殖腔拭子次之,粪便拭子最低(P<0·05),因此建议现场采样时应尽可能采集气管拭子标本;②陆禽标本检出率显著高于水禽标本(P<0·05),对病毒培养阳性的陆禽气管拭子检出率达80%(95%CI:70·6%~87·8%),对水禽气管拭子标本的检出率为38%(95%CI:26·9%~49·4%),可能与标本中病毒滴度高低有关;③有症状与无症状陆禽标本的检出率无显著差异。另外,H5-Dot试剂对333份非H5病毒气管拭子标本的特异性为99·4%(95%CI:97·9%~99·9%)。这些结果表明,H5-Dot是一种较为可靠的H5亚型禽流感病毒早期快速检测方法,在缺乏仪器设施和高素质专业技术人员的H5N1禽流感病毒防控第一线具有重要推广价值。更多还原

【Abstract】 Detection of 484 H5N1 positive field samples (trachea swabs, cloacae swabs and fecal swabs) obtained from terrestrial and aquatic poultry using a rapid antigen detection test, H5-HA(Ag) Dot-ELISA (H5-Dot), for direct and rapid detection of hemagglutinin antigen (Ag) of H5 subtype avian influenza virus was carried out. By using H5-Dot, it was shown that: ①The sensitivity was different in detecting field samples with varied sample sources, where trachea swab was higher than cloacae swab, and fecal swab was the lowest(P<0.05), which might be associated with variation of virus excretion titers. So, trachea swab is the best sample for detection of field H5 avian influenza virus by using this method; ②The sensitivity for H5-positive swab samples from terrestrial poultry was significantly higher than that from aquatic poultry(P<0.05), which was 80%(95%CI:70.6% - 87.8%)and 38%(95%CI:26.9% - 49.4%)for trachea swabs respectively; ③Sensitivity had no difference in detecting swab samples from diseased or asymptomatic terrestrial poultry. It also showed the nice specificity of 99.4% (95%CI:97.9% - 99.9%) for 333 non-H5 trachea swab samples.These results suggest that H5-Dot is an acceptable detection test for routine screening of H5N1 infection in the fields.更多还原