【摘要】 目的:动态观察鞘内注射(intrathecal,IT)吗啡对大鼠切口疼痛及脊髓Fos蛋白表达的影响。方法:雄性SD大鼠96只,分成4组,以累积疼痛评分观察大鼠行为学,以免疫组织化学法测定大鼠脊髓Fos蛋白表达。结果:在术后2 h、24 h、48 h时点,对照组大鼠的累积疼痛评分明显高于假手术组,术前和术后给药组评分较对照组均明显降低(P<0.01),但两组间差异均无统计学意义(P>0.05);术后第72 h,各手术组之间差异均无统计学意义(P>0.05);术后96 h和120 h,各组之间差异均无统计学意义(P>0.05)。在术后2 h,对照组大鼠Fos蛋白表达明显增多(P<0.01),而IT吗啡可明显抑制脊髓背角Fos蛋白表达,尤以术前给药为甚;术后24~120 h,各手术组间差异均无统计学意义(P>0.05)。结论:术前或术后单次IT吗啡,仅在术后早期有镇痛作用并能抑制Fos蛋白表达的增加。更多还原

【Abstract】 Objective:To observe the effects of intrathecal(IT) morphine on incisional pain and expression of c-fos in spinal cord of rats with postoperative pain.Methods:In a rat model of postoperative pain,the cumulative pain scores were used for evaluating pain behaviors.Immunohistochemistry technique was involved in observing the expression of c-fos in slices of spinal cord of rats.Results:In control group,cumulative pain scores were significantly higher than that in sham-operation rats 2 hours,24 hours and 48 hours after incision and at the same time,compared with that in control group,cumulative pain scores in treatment groups were significantly lower(P<0.01).There was no significant difference among treatment groups administered with drugs either before or after incision(P>(0.05)).Seventy-two hours after incision,there was no significant difference in cumulative pain scores among operative groups(P>(0.05)).From 96 hours to 120 hours after incision,there was no significant difference among all groups(P>0.05).IT morphine given either before or after incision decreased the number of Fos-LI neurons significantly in dorsal horn of spinal cord 2 hours after incision(P<(0.01)).IT morphine given before incision decreased the number of Fos-LIl neurons more effectively than that after incision.From 24 hours to 120 hours after incision,there was no significant difference among all operative groups(P>0.05).Conclusions:In a rat model of postoperative pain,IT morphine administered either before or after incision provides analgesia and inhibits the Fos protein expression only in early period after incision.更多还原