【摘要】 研究了转PEPC基因水稻及其原种在光抑制和光氧化条件下叶绿素荧光等参数的变化。结果表明,玉米PEPC基因导入水稻后,PEPC活性显著提高。不同基因型水稻在中午高光强高温条件下均发生光抑制现象,其中转PEPC基因水稻中午Fv/Fm、qP和PSⅡ降低较少,qN略为增加,光能转化为化学能的效率较高,热耗散较低,表现耐光抑制。经光氧化处理,转PEPC基因水稻叶绿素和蛋白含量下降较少,表现耐光氧化特性。同时转PEPC基因水稻Fv/Fm和qP下降较少,qN增加较多,可见其保持较高的光化学效率和增加热耗散提高耐光氧化能力。更多还原

【Abstract】 Changes of chlorophyll fluorescence parameters and other indexes in transgenic rice and its wild tpye were studied under photoinhibition and phooxidation conditions.Results showed that PEPC activity increased significantly after the introduction of PEPC gene from maize into rice.Photoinhibition of photosynthesis existed at noon in different genotypes under natural condition with high light and high temperature in which Fv/Fm,qP and PSⅡ reduced less and qN increased less in PEPC transgenic rice.Thus the efficiency of light energy transferring to chemical energy was higher and the thermal energy dissipation was lower.It was tolerant to photoinhibition.Under photooxidation,the content of chlorophyll and protein reduced less in PEPC transgenic rice,which demonstrated it was tolerant to photooxidation.At the same time,Fv/Fm and qP reduced less and qN increased more in PEPC transgenic rice,which showed that it enhanced tolerance to photooxidation through the higher efficiency of light energy transferring to chemical energy and the thermal energy dissipation.更多还原