【摘要】 研究多种因素对欧亚种酿酒葡萄品种“黑比诺”叶片、叶柄和茎段不定芽再生的影响。不同生长调节物质及浓度对其再生影响显著,其中以茎段在MS+BA 5.0 mg.L-1+NAA 0.1 mg.L-1培养基上再生效果最好,再生率为62.5%;叶柄在MS+BA 5.0 mg.L-1+IBA 0.1 mg.L-1培养基上再生率达到20%,而叶片始终未能诱导出芽。另外,暗培养时间及不同节位对茎段不定芽再生有明显影响。更多还原

【Abstract】 In this paper,adventitious bud regeneration from leaf,petiole and shoot explants of grape "Pinot noir" was studied with MS medium supplemented different combinations of BA,NAA and IBA.The results indicated that the best effect was obtained when stem explants were incubated on MS medium with BA 5.0 mg·L-1+NAA 0.1 mg·L-1,which reached 62.5%.But any adventitious buds were not formated from leaf explants on initiation mediun.The highest rate of stem explants regeneration reached 20% on MS medium with BA 5.0 mg·L-1+IBA 0.1 mg·L-1.In addition,it is evident that stem explants were incubated with dark-culture time and different sections.更多还原