【摘要】 小熊猫(Ailurus fulgens)是生活在陆地上的活化石,在类熊类哺乳动物的进化史中具有关键的作用,但近年来数量急剧下降,因此小熊猫的品种保护逐渐受到人们的重视。细胞的培养和冷冻保存已经成为种质资源保护的重要方法。本试验采取小熊猫的耳部组织,用组织培养法对小熊猫组织进行原代、继代培养并成功进行了冷冻和解冻处理,建立了耳部成纤维细胞系。并对培养的细胞进行了形态学观察,该细胞系的建立填补了小熊猫体细胞培养的空白,使得小熊猫种质资源在细胞水平上得到了保存。更多还原

【Abstract】 The red panda(Ailurus fulgens) is one of the earth′s living fossils.The evolutionary history of the red panda plays a pivotal role in the higher-level phylogeny of the arctoid carnivoran mammals.In China,such animal distributes in Sichuan,Yunan and Tibet Provinces but suffers from the amount reduction.Nowadays,the cryopreservation of somatic cells has become a useful method to store the important resource,and then accepted increasingly.In our present,the fibroblast cell line of red panda was successfully established by tissue culture,and then cryopreserved in liquid nitrogen.It is necessary to develop the feasible ways to preserve this species.更多还原