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Pseudomonas fluorescenssp-f铁载体高效液相凝胶色谱分析
Analysis of Siderophores from Pseudomonas fluorescens sp-f by Size-Exclusion High Performance Liquid Chromatography
【摘要】 利用高效液相凝胶色谱法对荧光假单胞菌所产铁载体进行了分析,结果显示高产铁载体P.fluorescens sp-f与P.fluorescens AB92001均可分泌3种铁载体,其中具有荧光的pyoverdine容易被细胞外的铁抑制。高效液相凝胶色谱法适用于假单胞菌铁载体的分析检测。
【Abstract】 Size-exclusion high performance liquid chromatography was used to analyze the siderophores from P.fluorescens.The results indicated that both over-siderophores producting P.fluorescens sp-f and P.fluorescens AB92001 could produce and release 3 kinds of siderophores,of which fluorescent pyoverdine responded sensitively to extracellular iron.Siderophores analysis with SE-HPLC was viable.
【关键词】 荧光假单胞菌;
铁载体;
高效液相凝胶色谱法;
【Key words】 Pseudomonas fluorescens; siderophores; size-exclusion high performance liquid chromatography(SE-HPLC);
【Key words】 Pseudomonas fluorescens; siderophores; size-exclusion high performance liquid chromatography(SE-HPLC);
【基金】 武汉市青年科技晨光计划(20015005051)
- 【文献出处】 氨基酸和生物资源 ,Amino Acids & Biotic Resources , 编辑部邮箱 ,2007年03期
- 【分类号】Q936-33
- 【被引频次】4
- 【下载频次】291