【摘要】 背景与目的:Mucin-1(MUC1)粘蛋白是一种肿瘤相关抗原,是肿瘤免疫治疗良好的分子靶点之一。本研究建立高表达MUC1的人食管癌裸鼠皮下移植瘤模型,为研究以MUC1为靶点的食管癌的生物治疗提供体内模型。方法:以体外培养的高表达MUC1的人食管癌细胞株EC-109接种于4～5周龄的BALB/c裸小鼠皮下,观察移植瘤生长情况,对移植瘤进行病理组织学检查,采用免疫组化方法检测移植瘤细胞增殖细胞核抗原(proliferating cell nuclearantigens,PCNA),采用流式细胞仪检测移植瘤细胞的细胞周期及MUC1的表达。结果:裸鼠皮下移植瘤成瘤率为86.0%,移植瘤具有与人恶性肿瘤相似的组织学和生物学特点,其平均PCNA标记指数为(63.5±3.6)%、S期细胞百分比(S-phase fraction,SPF)平均值为(37.6±3.7)%、MUC1的平均表达率为97.5%。结论:高表达MUC1的人食管癌裸鼠皮下移植瘤模型具有恶性肿瘤的生物学特性,可用于研究人食管癌的生物学特点,同时为以MUC1为靶点的食管癌的免疫治疗研究提供了良好的体内模型。更多还原

【Abstract】 BACKGROUND & OBJECTIVE: Mucin-1 (MUC1),a tumor-associated antigen,is an optional molecular target for antitumor immunotherapy protocols. This study was to establish a subcutaneous human esophageal cancer transplantation model with MUC1 high expression in nude mice that closely resembles the biological features of human esophageal cancer,and provide a in vivo model for MUC1-targeting immunotherapy of esophageal cancer. METHODS: Human esophageal carcinoma cell line EC-109 with MUC1 high expression was cultured,and subcutaneously transplanted into BALB/c athymic nude mice (4-5 weeks old). The growth status of transplanted tumor was observed. These subcutaneous tumors were examined histologically. The expression of proliferating cell nuclear antigen (PCNA) was detected by immunohistochemistry. Cell cycle and MUC1 expression of the transplanted tumor cells were analyzed by flow cytometry. RESULTS: Human esophageal carcinoma transplantation models with MUC1 high expression were successfully established in 6 of the 7 nude mice. The histological and biological characteristics of subcutaneous transplanted tumors were similar to those of human esophageal carcinoma. The mean PCNA label index of subcutaneous transplanted tumor cells was (63.5±3.6)%. The mean S-phase fraction of cell cycle was (37.6±3.7)%. The positive rate of MUC1 in subcutaneous transplanted tumor cells was 97.5%. CONCLUSION: Human esophageal carcinoma transplantation model with MUC1 high expression in nude mice is similar to human malignant tumors in biological characteristics,and can be used to investigate the biological characteristics of esophageal carcinoma,as well as provides an applicable animal model for research on MUC1-targeting immunotherapy of esophageal cancer.更多还原