【摘要】 目的:建立黄芪注射液浓缩液微乳中黄芪甲苷的含量测定方法。方法:采用(HPLC-ELSD)。色谱柱:Zorbax C18柱(4.6 mm×250 mm,5μm);流动相:乙腈∶水(36∶70);检测波长:206nm;灵敏度为0.08 AUFS,流速:1.0 ml/min;柱温:25℃。ELSD参数:漂移管温度为55℃,空气气压280 kPa。结果:黄芪甲苷在0.004 145 6~0.040 456 0 mg/ml时有良好的线性关系(r=0.999 1),平均回收率为96.53%,RSD为0.76%。结论:HPLC-ELSD法具有准确、简便、灵敏度高、无干扰而且重现性好的优点,适合用于黄芪注射液微乳中黄芪甲苷含量的测定。更多还原

【Abstract】 Objective:To establish a method for determining astragaloside Ⅳ in injection astragalus microemulsion.Methods: HPLC-ELSD was employed in the quantitative analysis of astragaloside Ⅳ in the sample.The separation was performed on Zorbax C18(4.6 mm×250 mm,5 μm) column with a mixture of acetonitrile-water(36∶70) as mobile phase.Wavelength:206 nm;response 0.08 AUFS.The flow rate was 1.0 ml/min.ELSD parameter: The temperature of drift tube for measurement was 55℃,air pressure:280 kPa,CAIN6.Results: The standard curve was linear in the range of 0.004 145 6~0.040 456 0 mg/ml.r=0.999 1.The average recovery was 96.53%,RSD: 0.76%.Conclusion: The method is simple,reliable,sensitive,free from interference and it have good reproductivity.Therefore it is fit to determine astragaloside Ⅳ in microemulsion.更多还原