【摘要】 目的建立甲硫氨酸含量的高效液相色谱测定方法。方法色谱柱为ODS-C18(250 mm×4.6 mm,5μm);流动相为甲醇-水(10∶90),流速为1.0 ml.min-1,检测波长为210 nm。结果甲硫氨酸在20-60 mg.L-1范围内,线性关系良好,回归方程:Y=195.32X+21.34,r=0.999 9。平均回收率99.57%(n=9),RSD=0.56%。结论该方法简便、重现性好、灵敏度高,专属性强,可以用作甲硫氨酸的含量测定。更多还原

【Abstract】 Aim To establish the method of determining the methionine tablets with HPLC.Methods The chromatographic column was ODS-C18(250 mm×4.6 mm,5 μm).The mobile phase was acetone alcohol-water(10∶90).The flow rate was 1.0 ml·min-1,and the detection wavelength was 210 nm.Results The linear range of methionine was in the range of 20～60 mg·L-1.The linear relation was good.The regression equation was Y =195.32X +21.34,r=0.999 9,and its average recovery was 99.57%(n=9),RSD=0.56%.Conclusion The method is reproducible,sensitive and specific.It can be used for determination of methionine.更多还原