【摘要】 目的探讨全自动尿沉渣分析仪和相差显微镜联合检测在鉴别肾源性血尿与非肾源性血尿中的应用价值。方法利用Sysmex UF-100尿沉渣分析仪和Olympus CX40相差显微镜联合检测79例不同来源血尿标本,通过检测和鉴别尿红细胞数、尿红细胞形态大小及尿红细胞前向散射光强度(RBC-Fsc),帮助判断血尿的性质及来源。结果43例肾源性血尿,尿RBC畸形率为85.39%(>80%),当RBC-Fsc≤70ch时,敏感性91.5%,特异性84.6%,诊断符合率93.3%;36例非肾源性血尿,RBC畸形率为13.8%,RBC-Fsc通常>84ch;其中11例混合性血尿,RBC畸形率为67.4%,126ch>RBC-Fsc>84ch。结论UF-100尿沉渣分析仪与CX40相差显微镜联合检测分析尿RBC数、尿RBC形态、RBC-Fsc及提供的定量信息和散点图、直方图数据等指标,能客观、准确地鉴别和诊断血尿的来源。更多还原

【Abstract】 Objective To evaluate the values of combined detection for distinguishing glomerular hematuria from nonglomerular hematuria by Olympus CX40 phase contrast microscope and Sysmex UF-100 automated flow cytometer.Methods The count and morphology of urinary red cell(RBC) in 79 urine samples from hematuria patients were measured by phase contrast microscope and urinary RBC mean forward scattered light intensity (RBC-MFsc) were measured by Sysmex UF-100 automated flow cytometer simultaneously. Results In 43 patients with glomerular hematuria the percentage of deformed red cells was 85.39% (>80%) . When the cut-off value of RBC-MFsc was defined as less than or equal to 70 ch for glomerular hematuria, the sensitivity was 91.5%, specificity was 84.6% and accuracy was 93.3%. In 36 patients with nonglomerular hematuria, the percentage of deformed red cells was 13.8% and RBC-MFsc was more than 84 ch. In 11 patients with mixed hematuria, the percentage of deformed red cells was 67.4%, and RBC-MFsc was between 84 ch and 126 ch. Conclusion The combined examination including quantity, morphology and MFsc of urinary red cells, and therefore the indexes of scatter plot and histogram provided may be able to disclose and diagnose the source of hematuria more objectively and accurately.更多还原