【摘要】 通过三亲本杂交将花生根瘤菌吸氢基因的重组质粒pZ55(Tcr)转入野生型的大豆、绿豆等根瘤菌菌株(Hup-、Apr),研究结果表明,在自生条件下,诱导大豆和绿豆根瘤菌吸氢活性的表达,实现了花生根瘤菌吸氢基因的种间转移。在豌豆和四季豆根瘤菌中却不能表达吸氢活性,提示外源吸氢基因的表达受到受体菌株的调控。更多还原

【Abstract】 Interspecies transfer of hup gene by triparental mating from R.arachis to R.vaigaris,R.aeruea,R.japonicum and R leguminusarum was carried out in this paper.The transconjugants were confirmed by plasmid extraction and H2-uptake activity measurement.High H2-uptake activity was detected only in transconjugants R.aerues and R.japonicum.No activity was detected in R.vaigaris and R.Leguminusarum strains.更多还原