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紫茉莉茎提取物及其萃取物对菜粉蝶幼虫的生物活性

Bioactivity of Extracts and Fractions from Stem of Mirabilis jalapa L. to Pieris rapae L.

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【作者】 彭跃峰鲁红学刘铁铮

【Author】 PENG Yue-feng et al (Agricultural College,Yangtze University,Jingzhou,Hubei 434025)

【机构】 长江大学农学院长江大学农学院 湖北荆州434025湖北荆州434025

【摘要】 用饲喂法、叶片夹毒法、叶碟法测定了紫茉莉茎的3种极性溶剂(石油醚、氯仿和乙醇)提取物及萃取物对菜粉蝶幼虫的毒杀活性、胃毒作用、非选择性拒食活性。结果表明,紫茉莉茎氯仿提取物的毒杀与拒食效果最好,处理第5天后的校正死亡率为89.20%,取食第3天与第5天的拒食率分别为96.80%与99.67%;也测定了紫茉莉茎氯仿提取物(简称MSCE)各萃取物(正己烷、氯仿和水萃取物)对4龄菜粉蝶幼虫的生物活性。结果表明,MSCE的正己烷萃取物第5天与第7天的毒杀效果最好,其校正死亡率分别为79.80%与78.90%;其对4龄菜粉蝶幼虫的胃毒作用明显的强于其他各萃取物。

【Abstract】 The poison activity,stomach toxicity action and nonselective food refusal activity of extracts and fractions obtained by extracting the stem of Mirabilis jalapa L against the 4th instar larvae of Pieris rapae L. with 3 polarity solvents(petroleum ether,chloroform and ethanol)were determined by the methods of feeding,leaf-sandwich and leaf dish.The results showed that the chloroform extracts had the best poison effects and food refusal action,with the corrected mortality of 89.20% after treatment at 5th day,and the food refusal rates of 96.80% and 99.67% after getting food at 3rd and 5th day respectively.Determination on the bioactivity of 3 fractions(nexane,chloroform and water fraction)of the chloroform fractions from stem of Mirabilis jalapa L (MSCE) against 4th instar larvae of Pieris rapae L.indicated that the nexane fraction of MSCE possessed the best poison effects,with the corrected mortality of 79.80% and 78.90% after treatment at 5th and 7th day respectively.Its stomach poison action was remarkably higher than other fractions.

【关键词】 紫茉莉提取物萃取物菜粉蝶生物活性
【Key words】 Mirabilis jalapa L.ExtractFractionPieris rapae L.Bioactivity
【基金】 长江大学科学基金项目(HNIDI200303)
  • 【文献出处】 安徽农业科学 ,Journal of Anhui Agricultural Sciences , 编辑部邮箱 ,2007年13期
  • 【分类号】S482.3
  • 【被引频次】22
  • 【下载频次】175
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