【摘要】 在确定合适碳源的基础上,研究比较不同蔗糖浓度下铁皮石斛原球茎生长和多糖的积累情况。结果表明,在葡萄糖、果糖、蔗糖、乳糖4种碳源中,蔗糖和葡萄糖对原球茎生长和多糖积累的影响优于乳糖和果糖。在蔗糖浓度为30~60 g/L时,浓度越高,原球茎生长受到抑制越大,而且生长周期延长,但多糖含量增加越快,多糖含量越高;当蔗糖浓度超过60 g/L时,蔗糖表现出明显的抑制效应;适合多糖积累的蔗糖起始浓度为30 g/L时,有利于原球茎的生长,最大干重量为17.93 g/L,多糖最高含量为208.7 mg/g,培养40 d时,其产量为3679.6 mg/L。更多还原

【Abstract】 On the basis of the determination of the suitable carbon source,the biomass and the accumulation of polysaccharide from protocorms of Dendrobium candium were studied under different sucrose concentration.The results showed that sucrose and glucose were better than fructose and lactose for protocorms growth and polysaccharide accumulation at the concentration of 30～60 g/L,the inhibitory effect on the growth of protocorms increased,and the growth cycle was becoming longer with sucrose concentration increasing.But the content of polysaccharide increased.Moreover,the sucrose presented inhibition effect on protocorms growth while its concentration was above 60 g/L.The optimum concentration of sucrose for accumulation of polysaccharides was 30 g/L.The maximum biomass of protocorms was 17.93 g/L.The content of polysaccharide reached 208.7 mg/g.And the yield of polysaccharide was 3 679.6 mg/L after 40 day’s incubation.更多还原