【摘要】 通过活化酯法和混合酸酐法,用牛血清蛋白(BSA)和卵清蛋白(OVA)合成2,4-D免疫抗原和包被抗原,再分别采用紫外分光光度法和酶联免疫吸附法(ELISA)进行鉴定。结果是:2种方法合成的完全抗原中2,4-D和蛋白质的偶联比分别为24∶1和17∶1,抗血清效价为1.28×104~2.56×104。更多还原

【Abstract】 In the lab experiment,through using active ester method and mixed anhydride method,the immunity antigens and coating antigens of herbicide 2,4-dichlorphenoxyacetic acid(2,4-D) was synthesized by bovine serum albumin(BSA) or ovalbumin(OVA) and identified by ultraviolet spectrophotometry and ELISA.The results showed that the ratio of 2,4-D∶BSA was 24∶1,and that of 2,4-D∶OVA was 17∶1,respectively.The anti-2,4-D polyclonal antibodies were obtained by using the artificial immune antigen(2,4-D-BSA) to immune in the New Zealand rabbits.The titer of anti-serum was from 1.28×104 to 2.56×104.更多还原