【摘要】 主要介绍了umu测试方法的实验原理和在环境科学中的研究现状。umu实验(即SOS/umu实验)起源于20世纪80年代中期,是用于检测环境致突变的短期筛选实验。它基于环境诱变物质损伤DNA后诱导SOS反应,表达umu基因,进而生成具有β-半乳糖苷酶活性原理设计而成。通过检测此酶的活性可确定受试物引起的DNA损伤程度。因该方法具有简单易操作、检出率高、廉价等优点,因而被广泛应用于遗传毒理学研究、酶代动力学分析、环境监测与质量评估以及药效学评价和新药筛选等方面。更多还原

【Abstract】 The principles of umu test and application status in environmental science were studied.Umu test(umu/SOS test) developed from the mid-1980s was an effective short-term assay to determine the genotoxic activity of a chemical.This method was based on one principle,which was when a chemical induced DNA damage,the umuC gene would express and induce β-galactosidase.The genotoxic activity of a chemical could be detected by β-galactosidase activity determination.Based on the advantages of umu test including simpler procedure,rapid sensitive and economical efficiency,umu test was applied in many scientific aspects such as metabolizing dynamics analysis of enzyme,water quality assessment and new medicine selection.更多还原