【摘要】 为探讨黑曲霉固态发酵酸性磷酸酯酶(ACP)的产酶条件,首次采用固态培养的方法,在对黑曲霉3350产酸性磷酸酯酶的培养基组成和培养条件单因素研究的基础上,设计了Plackett-Burman试验选取了3个主要影响因素,并进行响应面分析。结果表明,黑曲霉3350产酸性磷酸酯酶的最适培养基和发酵条件为:麸皮8.0 g,蔗糖2.0 g,豆饼粉1.11 g,水13.9 ml,土温-801.0 ml/kg,NH4NO320 g/kg,KH2PO42.5 g/kg,自然pH值;32℃固体静置培养120 h,期间翻曲2~3次。在该条件下菌株最高产酶活性为154 IU/g干曲,比优化前产酶提高88%,与液态发酵周期(9 d)相比缩短了44%。更多还原

【Abstract】 The research on the nutrition ingredients and fermentation conditions on the production of acid phosphatase by Aspergillus niger 3350 showed that the optimal fermentation medium and condition contained 8.0 g wheat bran,2.0 g sucrose, 1.11g soybean flour,13.9 ml H₂O,soil temperature-80 of 1.0 ml/kg,20 g/kg, KH₂PO₄ 2.5 g/kg,CaCl₂ 1.0 g/kg,MgSO₄ 1.0 g/kg,nature pH,solid culture for 20 h at 32 ℃,turning over the aspergillus 2 times.Under these conditions,the highest acid phosphatase activity was 154 IU/g dry matter,being 88% higher than acid phosphatase production before optimization.更多还原