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禽霍乱蜂胶灭活疫苗研制中的铁螯合剂适宜浓度确定

Development of a Novel Broad-spectrum Propolis Adjuvant Inactivated Vaccine against Fowl Cholera

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【摘要】 以含4%鸡血清的营养琼脂为培养基,进行了铁螯合剂2,2’-联吡啶对禽多杀性巴氏杆菌C48-3株的抑菌试验。结果表明,对于含4%鸡血清的营养琼脂培养基,铁螯合剂2,2’-联吡啶的合适添加量为200μmol/L。

【Abstract】 Nutritious agar supplemented with 4% chicken serum was used as culture media.Suppressive effect of iron-chelator 2,2’-dipyridyl on pasteurella multocida strain C48-3 was studied in order to find an easy method to confirm the appropriate concentration of iron-chelator supplemented in a specific culture media to induce expression of cross-protective factor(CPF) by pasteurella multocida.The result indicated that with this method,an appropriate concentration of iron-chelator could be found out for specific culture media to induce expression of CPF.As for nutritious agar culture media supplemented with 4% chicken serum,the appropriate concentration of 2,2’-dipyridyl was 200 μmol/L.

【基金】 安徽省教育厅自然科学研究项目“新型广谱禽霍乱蜂胶灭活疫苗的研制(2006KJ416B)”
  • 【文献出处】 安徽农业科学 ,Journal of Anhui Agricultural Sciences , 编辑部邮箱 ,2007年05期
  • 【分类号】S853.7
  • 【被引频次】2
  • 【下载频次】81
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